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|Specimen:||Whole Blood/Serum/Plasma||Testing Time:||5-15 Minutes|
|Shelf Life:||24 Months||Application:||Cattle Brucella|
Specimen: Whole Blood/Serum/Plasma
Cattle & Goat Brucella Rapid Diagnostic Test (W/S/P) is a rapid visual immunoassay for the qualitative, presumptive detection of antibody of brucella in bovine and goat blood specimens at the cut-off concentrations listed below:
This test strip is based on fast immuno-chromatography technique to detect Brucella Ab in bovine and goat serum, plasma and whole blood qualitatively. When testing, Brucella Ab in sample combine with antigen coated by colloid gold forming complexes, moving forward to the other head. When reaching the T-line, the specific antigen on the membrane capture the complexes and appear a T line, C line appears, means the test is valid. The T line appears, means there is Brucella Ab in the sample.
Collect anticoagulated whole blood, use for that very day;
Collect blood, put at 37℃ for 1-2h, take the supernatant, centrifuge at 1500r/min for 10min, separate the serum. The serum can store for 2-3 days at 4℃, for long term storage, store at -20℃ in frozen.
INTERPRETATION OF RESULTS
(Please refer to the illustration above)
NEGATIVE:* A colored line appears in the Control region (C) and colored lines appears in the Test region (T). This negative result means that the concentrations in the urine sample are below the designated cut-off levels for a particular drug tested.
*NOTE: The shade of the colored lines(s) in the Test region (T) may vary. The result should be considered negative whenever there is even a faint line.
POSITIVE: A colored line appears in the Control region (C) and NO line appears in the Test region (T). The positive result means that the drug concentration in the urine sample is greater than the designated cut-off for a specific drug.
INVALID: No line appears in the Control region (C). Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for Control line failure. Read the directions again and repeat the test with a new test card. If the result is still invalid, contact your manufacturer.
A procedural control is included in the test. A line appearing in the control region
(C) is considered an internal procedural control. It confirms sufficient specimen volume, adequate membrane wicking and correct procedural technique.
Control standards are not supplied with this kit. However, it is recommended that positive and negative controls be tested as good laboratory practice to confirm the test procedure and to verify proper test performance.
The specimen correlation study was performed on 300 Blood specimens. 82 positive urine specimens and 218 negative urine specimens were confirmed by GC/MS. These specimens were randomized and tested using the Cattle & Goat Brucella Rapid Diagnostic Test. Specimens were rated as either positive or negative at 5minutes. The test results are shown in Table 1.
Table 1: Specimen Correlation
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|Email:||Jerry @ newlifebiotest .com|