Tuberculosis remains an important sovio-eoonomical and medical problem throughout the world, According to the Centers for Control, the incidence of TB is expected to increase from 7.5 million cases per year in 1995 to 11.9 million in 2005. The ease fatality rate is estimated at 55% for untreated people and 15% for the treated patients.The commonly used diagnostic tests for the tuberculosis such as sputum examination of acid fast bacilli, culture of sputum or other fluids, the tuberculin skin test and radiological investigations do not achieve the required diagnostic sensitivity. TB RST is a CIA for the detection of TB antibody in human whole blood , serum or plasma. Specific antigens are pre-coated onto membrane as a capture reagent on the test region(T). During the test, specimen is allowed to react with specific antigen, which conjugated with colloidal gold firstly.And then chasing buffer mixed with colloidal gold will flow onto the membrane. If TB antibody present, a pink colored band will develop on the test region of membrane. Absence of this pink colored band in the test region suggests a negative result. To serve as a rocedural control, a pink colored band in the C region will always appear regardless the presence of TB antibody.