Custom Monoclonal Antibody Anti-Alpha LH Luteinising Hormone

Custom Mouse Monoclonal Antibody Anti-Alpha LH (luteinising hormone)

Description:

LH is a tropic hormone, which modulates the secretory activity of other endocrine glands. It is produced in the anterior hypophysis of the pituitary gland. The glycoprotein hormone, LH, like follicle stimulating hormone and thyroid stimulating hormone, is composed of a common alpha-sububit but also a specific beta-subunit, which characterizes each of these hormones.

Comments: This antibody is suitable for staining of formalin-fixed, paraffin-embedded tissues.

Mol. Wt. of Antigen: 14kDa (β), 28kDa(α/β)

Species Reactivity: Human. Others not tested.

Ig Isotype: IgG1/ κ

Immunogen: LH purified from human pituitary gland

Applications and Suggested Dilutions:

Immunohistology (Formalin/paraffin) (Ab at 1:2000 for 20 min using LV’s UltraVision LP Systems, at 1:300 to 1:500 for 30 min with LV’s UltraVision)

* [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.]

The optimal dilution for a specific application should be determined by the investigator.

Positive Control: Anterior pituitary

Cellular Localization: Cytoplasmic

Storage and Stability: Store vial at 40C. When stored at 2-80C, this antibody is stable for 24 months.

Supplied As: 200µg/ml of antibody purified from ascites fluid by Protein G chromatography. Prepared in 10mM PBS, pH 7.6, with 0.2% BSA and 15mM sodium azide.

Limitations and Warranty: Our products are intended FOR RESEARCH USE ONLY and are not approved for clinical diagnosis, drug use or therapeutic procedures. No products are to be construed as a recommendation for use in violation of any patents. We make no representations, warranties or assurances as to the accuracy or completeness of information provided on our data sheets and website. Our warranty is limited to the actual price paid for the product. It is not liable for any property damage, personal injury, time or effort or economic loss caused by our products.

Material Safety Data: This product is not licensed or approved for administration to humans or to animals other than the experimental animals. Standard Laboratory Practices should be followed when handling this material. The chemical, physical, and toxicological properties of this material have not been thoroughly investigated. Appropriate measures should be taken to avoid skin and eye contact, inhalation, and ingestion. The material contains 0.09% sodium azide as a preservative. Although the quantity of azide is very small, appropriate care should be taken when handling this material as indicated above. The National Institute of Occupational Safety and Health has issued a bulletin citing the potential explosion hazard due to the reaction of sodium azide with copper, lead, brass, or solder in the plumbing systems. Sodium azide forms hydrazoic acid in acidic conditions and should be discarded in a large volume of running water to avoid deposits forming in metal drainage pipes.