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Purified Mouse Monoclonal Antibody Anti-Beta LH (luteinising hormone)

Description:

LH is a tropic hormone, which modulates the secretory activity of other endocrine glands. It is produced in the anterior hypophysis of the pituitary gland. The glycoprotein hormone, LH, like follicle stimulating hormone and thyroid stimulating hormone, is composed of a common alpha-subunit but also a specific betasubunit, which characterizes each of these hormones. LH producing adenomas represent approximately 6% of pituitary adenomas. LH / FSH secreting adenomas have slow growth and at the time of diagnosis are generally large, though rarely associated with high serum levels of gonadotropins. LH secreting adenomas are only rare. Null adenomas and oncocytomas do not show clinically or biochemically signs of hormone production but in the pituitary they tissue show focally expression of LH, FSH, TSH and alpha subunit.

Composition: anti-human LH mouse monoclonal antibody purified and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Intended use : Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting

Ig isotype: mouse IgG1/k

Immunogen: LH purified from human pituitary gland

Species reactivity: In vitro diagnostics in humans. Not tested in other species

Applications and Suggested Dilutions:

Immunohistology (Formalin/paraffin) (Ab at 1:2000 for 20 min using LV’s UltraVision LP Systems, at 1:300 to 1:500 for 30 min with LV’s UltraVision)

* [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.]

The optimal dilution for a specific application should be determined by the investigator.

Positive Control: Anterior pituitary

Cellular Localization: Cytoplasmic

Storage and Stability: Store vial at 40C. When stored at 2-80C, this antibody is stable for 24 months.

IHC recommended procedure:

1. 4µm thick section should be taken on charged slides; dry overnight at 60ºC - Deparaffinise, rehydrate and HIER (heat induced epitope retrieval)
2. boil tissue in the Pt Module using Master Diagnóstica EDTA buffer pH82 for 20 min at 95ºC. Upon completion rinse with 3-5 changes of distilled or deionised water followed by cooling at RT for 20 min.
3. Endogenous peroxidase block - Blocking for 10 minutes at room temperature using peroxidase solution (ref. MAD-021540Q-125).
4. Primary antibody: incubate for 1 minute [The antibody dilution (when concentrated) and protocol may vary depending on the specimen preparation and specific application. Optimal conditions should be determined by the individual laboratory].