Gold Colloidal Myoglobin Blood Test Home High Accuracy Kit Qualitative Detection

One step Myoglobin Rapid Diagnostic Test, detection of Myoglobin, gold colloidal method,quickly and easily

Intended Use:

The Myoglobin Rapid Test Cassette (Whole Blood/Serum/Plasma) is a rapid chromatographic immunoassay for the qualitative detection of human Myogobin in whole blood, serum or plasma as an aid in the diagnosis of myocardial infarction (MI).

Summary:

Myoglobin (MYO) is a heme-protein normally found in skeletal and cardiac muscle with a molecular weight of 17.8kDa.It constitutes about 2 percent of total muscle protein and is responsible with transporting oxygen within the muscle cells.1 When the muscle cells are damaged, Myoglobin is released to the blood rapidly due to its relatively small size. Following the death of tissue associated with MI, Myoglobin is one of the first markers to rise above normal levels. The level of Myoglobin increases measurably above baseline within 2-4 hours post-infarct, peaking at 9-12 hours and returning to baseline within 24-36 hours.2,3 A number of reports suggest the measurement of Myoglobin as a diagnostic aid in confirming the absence of myocardial infarction with negative predictive values of up to 100% reported at certain time periods after onset of symptoms.4 The Myoglobin Rapid Test Cassette (Whole Blood/Serum/Plasma) is a simple test that utilizes a combination of anti-Myoglobin antibody coated particles and capture reagents to qualitatively detect Myoglobin in whole blood, serum or plasma. The minimum detection level is 50ng/mL.

Test Principle:

 

The Myoglobin Rapid Test Cassette (Whole Blood/Serum/Plasma) is a qualitative, membrane based immunoassay for the detection of Myoglobin in whole blood, serum or plasma. The membrane is pre-coated with specific capture antibodies in the test line regions of the test. During testing, the whole blood, serum or plasma specimen reacts with the particle coated with specific antibodies. The mixture migrates upward on the membrane chromatographically by capillary action to react with specific capture reagents on the membrane and generate a colored line. The presence of this colored line in the specific test line region indicates a positive result, while its absence indicates a negative result. To serve as a procedural control, a colored line will always appear in the control line region indicating that proper volume of specimen has been added and membrane wicking has occurred.

TEST PROCEDURE

1. Bring the pouched test device to room temperature(15-30℃) prior to testing. Do not

open the pouch until ready to begin testing.

2.  Remove the device from the sealed pouch and lay it on a flat and dry surface.

3. Open the tube with the diluted sample .Transfer 3 drops of mixed specimens to sample well. Start the timer.

4. Wait for the colored lines to appear. The result should be read at 5 minutes. Do not interpret the results at 10 minutes.

INTERPRETATION OF RESULTS

POSITIVE:* Two lines appear. One colored line should be in the control line region (C) and another apparent colored line should be in the test line region (T).

*NOTE: The intensity of the color in the test line region (T) will vary depending on the concentration of TP antibodies present in the specimen. Therefore, any shade of color in the test line region (T) should be considered positive.

NEGATIVE: One colored line appears in the control line region (C). No line appears in the test line region (T).

INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test with a new test. If the problem persists, discontinue using the test kit immediately and contact your local distributor.