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Product Details:
Payment & Shipping Terms:
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| Method: | Elisa | Format: | 96T/KIT |
|---|---|---|---|
| Valid: | 24 Month | Accuracy: | 99.90% |
| Sensitivity: | 99.89% | Application: | Rubella IgM |
| High Light: | rapid elisa test,elisa assay kit |
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The Rubella IgM ELISA test , high accuracy, Elisa Sandwich method, for quantitative measurement
Product Name: The Rubella IgM ELISA test
Intended Use:
The Rubella IgM ELISA Test System is designed for the qualitative and/or quantitative detection of IgM antibodies to rubella virus in human serum. The test system is intended to be used to evaluate single sera for immune status or paired sera to demonstrate seroconversion, and is for in vitro diagnostic use.
Summary:
Rubella is an exanthematous viral disease of children and young adults. It is a self-limited and benign disease characterized by fever, mild upper respiratory symptoms, erythematous rash and suboccipital lymphodenopathy. Rubella can be a very serious disease early in pregnancy leading miscarriages or birth defects up to 85% of cases. Reinfection occurs more frecuently in vaccinated that in naturally immune individuals. The majority of these reinfections occur without symptoms. Rubella reinfections during pregnancy rarely results in transmission of the virus to the unborn child. Antibodies appear at the begining of the disease and initially both IgG and IgM can be detected. IgG antibodies usually persist throughout life. IgM antibodies IgM do not persist beyong 8 weeks.
Regents Provided
| Materials Provided | 96 Tests |
| Microwells coated with Rubella antigen | 12 x 8 x 1 |
| Sample Diluent: 1 bottle (ready to use) | 22 mL |
| Calibrator: 1 val (ready to use) | 1 mL |
| Positive Control: 1 vial (ready to use) | 1 mL |
| Negative Control: 1 vial (ready to use) | 1 mL |
| Enzyme conjugate: 1 bottle (ready to | 12 mL |
| use) | |
| TMB Substrate: 1 bottle (ready to use) | 12 mL |
| Stop Solution: 1 bottle (ready to use) | 12 mL |
| Wash concentrate 20x: 1 bottle | 25 mL |
TEST PRINCIPLE
The ELISA method is based upon the capture of IgM in the sample with anti-IgM antibodies adsorbed on the polystyrene surface. Unbound immunoglobulins are washed off. Then the antigen labeled with peroxidase react with the IgM captured, and the unbound is eliminated by washing; bound antigen is developed with the aid of a substrate solution (TMB) to render a blue coloured soluble product which turns into yellow after adding the acid stopping solution.
Results
Calculations
Example of typical results: Calibrator mean OD = 0.8 Calibrator Factor (CF) = 0.5 Cut-off Value = 0.8 x 0.5 = 0.400 Positive control O.D. = 1.2 Ab Index = 1.2/0.4 = 3 Patient sample O.D. = 1.6 Ab Index = 1.6/0.4 = 4.0
| INDEX | INTERPRETATION |
| < 9 | Negative |
| 9-11 | Equivocal |
| >11 | Positive |
Samples with equivocal results must be retested and/or a new sample obtained for confirmation.
| ORIENT NEW LIFE MEDICAL CO., LTD. | |
| Contact: | Jerry Meng |
| Email: | Jerry @ newlifebiotest .com |
| Tel. | +86 18657312116 |
| SKYPE | enetjerry |
Contact Person: Jerry Meng
Tel: +8618657312116
