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96 Wells HIV Antibody Test Kit Biotin Working Liquid Quantitative Measurement

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China Orient New Life Medical Co.,Ltd. certification
China Orient New Life Medical Co.,Ltd. certification
We are satisfied with your quality & services so far. The HIV 1/2 antibody Test performance is perfect.

—— Katrina

I have to say that New Life provides high quality products. The FOB, Troponin I, HIV, HCV, Drug Abuse test all performed very well in our market.

—— Clemen

Your Dengue IgG/IgM and Malaria test perfromed well. Now we want to try your Filariasis test in our market.

—— Primo

96 Wells HIV Antibody Test Kit Biotin Working Liquid Quantitative Measurement

96 Wells HIV Antibody Test Kit  Biotin Working Liquid Quantitative Measurement
96 Wells HIV Antibody Test Kit  Biotin Working Liquid Quantitative Measurement

Large Image :  96 Wells HIV Antibody Test Kit Biotin Working Liquid Quantitative Measurement Get Best Price

Product Details:
Place of Origin: China
Brand Name: New Life
Certification: ISO, CE
Model Number: 96 wells
Payment & Shipping Terms:
Minimum Order Quantity: 10 kit
Packaging Details: 96T/kit
Delivery Time: 20-35days
Payment Terms: T/T, Western Union
Supply Ability: 100000kit/month
Detailed Product Description
Method: Elisa Format: 96T/KIT
Valid: 24 Month Accuracy: 99.84%
Sensitivity: 99.83% Application: HIV 1/2 Antibody Elisa
High Light:

elisa diagnostic kits


elisa assay kit

HIV 1/2 antibody elisa test kitt,96wells/kit, high accuracy Elisa Sandwich method, for quantitative measurement





Materials provided with the kit:


Coated Microtiter Plate 1 bag 12*8
Conjugate 1vial 10.5mL
Biotin Working Liquid 1vial 3mL
Wash Buffer Concentrate (40*) 1vial 20mL
Substrate A 1vial 7mL
Substrate B 1vial 7mL
Stop Solution 1vial 6mL
Negative Control 1vial 1mL
Positive Control 1vial 1mL
Closure Plate Membrane 3sheet  


Note: different batches of reagent kit, and different component can not be exchanged for use. Once open, stable for 3 months at 2-8°C.





The Anti-HIV 1+2 ELISA is a qualitative enzyme immunoassay for the in vitro detection of Human

Immunodeficiency Virus (HIV) infection, based on the detection of antibodies to HIV-1 and HIV-2 in human serum or plasma. It is intended for screening of blood donors and diagnosing patients related to infection with HIV.




1. Prepare Reagents: Dilute 1 volume of Concentrated Washing Buffer with 19 volumes of distilled water,

mix well.


2. Add Samples: Open the foil pouch and remove the Microplate. Set up 1 well as Blank, 2 wells as negative control, 2 wells as positive control. After dispensing 50ul of Sample Diluent , dispense 50 ul of sample or negative control or positive control to the respective wells. Gently vibrating the plate.


3. Incubate: Cover the Microplate with plate cover and incubate the Coated Microplate in a thermostat-controlled water-bath or microplate incubator at 37 for 60 minutes.


4. Wash the Plate: Remove the plate cover. Aspirate the contents of all wells. Fill the wells with the diluted

washing buffer ( 10~20 seconds to soak) then aspirate again. Repeat the procedure for 5 times. Make sure that the rest volume is minimal, by tapping plate onto absorbent paper.


5. Add Conjugate: Add 100ul of conjugate to each well (except the blank well).


6. Incubate: Cover the Microplate and incubate the plate at 37 for 30 minutes.


7. Wash the Plate: Repeat the wash procedure as in step 4.


8. Add Substrate: Add 50ul of Substrate Solution A and 50 ul of Substrate Solution B to each well, mix well.


9. Stop reaction: Add 50ul Stop Solution to each well, mix well.


10. Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should


Name HIV 1,2 ELISA Test
Full name Human Immunodeficiency Viruses Type 1,2 Antibody (HIV1,2) ELISA Test Kit
Category Name HIV ELISA kit
Test 96 Tests
Method ELISA: Enzyme Linked Immunosorbent Assay
Principle ELISA: Sandwich; Double antigen
Detection Range Qualitative: Positive, negative and cutoff calibrators
Sample 100 uL Serum or plasma
Specificity 99.89%
Sensitivity 99.95%
Total Time 15 Min
Shelf Life 12 Months from the manufacturing date



Contact: Jerry Meng
Email: Jerry @ newlifebiotest .com
Tel. +86 18657312116
SKYPE enetjerry

Contact Details
Orient New Life Medical Co.,Ltd.

Contact Person: Jerry Meng

Tel: +8618657312116

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