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HBeAg ELISA test detect Hepatitis B E antigen,96wells/kit, Elisa Sandwich method, for quantitative measurement
1. HBeAg EIA is a qualitative enzyme immunoassay for the detection of HBeAg
2. human serum or plasma.
3. in vitro diagnostic kit
This kit uses “ sandwich” ELISA method in which, polystyrene microwell strips are pre-coated with monoclonal antibodies specific to HBeAg. Patient’s serum or plasma sample is added to the microwell together with a second monoclonal antibody conjugated to horseradish peroxidase (HRP-Conjugate).During incubation, the specific immunocomplex formed in case of presence of HBeAg in the sample is captured on the solid phase. After washing to remove sample and unbound HRP-Conjugate, Chromogen solutions containing Tetramethylbenzidine (TMB) and urea peroxide are added into the wells. In presence of the antibody-antigen-antibody(HRP) “sandwich “ complex, the colorless Chromogens are hydrolyzed by the bound HRP conjugate to a blue-colored product. The blue color turns yellow after stopping the reaction with sulfuric acid. The amount of color intensity can be measured and is proportional to the amount of antigen captured in the wells, and to the sample respectively. Wells containing samples negative for HBeAg remain colorless.
This kit is an enzyme -linked immunosorbent assay (ELISA) for qualitative detection of hepatitis B virus e antigen (HBeAg) in human serum or plasma. It is intended for use in clinical laboratories for diagnosis and management of patients related to infection with hepatitis B virus. HEPATITIS B VIRUS E ANTIGEN ELISA. One-Step Incubation, Double Antibody Sandwich Principle
Materials provided with the kits:
Materials required but not provided:
|ORIENT NEW LIFE MEDICAL CO., LTD.|
|Email:||Jerry @ newlifebiotest .com|
Contact Person: Jerry Meng