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HBeAg Elisa Assay Kit Polystyrene Microwell Strips Require Distilled Water

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HBeAg Elisa Assay Kit Polystyrene Microwell Strips Require Distilled Water

China HBeAg Elisa Assay Kit Polystyrene Microwell Strips Require Distilled Water supplier
HBeAg Elisa Assay Kit Polystyrene Microwell Strips Require Distilled Water supplier HBeAg Elisa Assay Kit Polystyrene Microwell Strips Require Distilled Water supplier

Large Image :  HBeAg Elisa Assay Kit Polystyrene Microwell Strips Require Distilled Water

Product Details:

Place of Origin: China
Brand Name: New Life
Certification: ISO, CE
Model Number: 96 wells

Payment & Shipping Terms:

Minimum Order Quantity: 10 kit
Packaging Details: 96T/kit
Delivery Time: 20-35days
Payment Terms: T/T, Western Union, MoneyGram
Supply Ability: 100000kit/month
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Detailed Product Description
Method: Elisa Format: 96T/KIT
Valid: 24 Month Accuracy: 99.988%
Sensitivity: 99.987% Application: HBeAg ELISA

HBeAg ELISA test detect Hepatitis B E antigen,96wells/kit, Elisa Sandwich method, for quantitative measurement

 

1. HBeAg EIA is a qualitative enzyme immunoassay for the detection of HBeAg
2. human serum or plasma.
3. in vitro diagnostic kit

 

Certificate
GMP
SFDA
ISO13485
ISO9001:2008

 

SUMMARY

 

 

This kit uses “ sandwich” ELISA method in which, polystyrene microwell strips are pre-coated with monoclonal antibodies specific to HBeAg. Patient’s serum or plasma sample is added to the microwell together with a second monoclonal antibody conjugated to horseradish peroxidase (HRP-Conjugate).During incubation, the specific immunocomplex formed in case of presence of HBeAg in the sample is captured on the solid phase. After washing to remove sample and unbound HRP-Conjugate, Chromogen solutions containing Tetramethylbenzidine (TMB) and urea peroxide are added into the wells. In presence of the antibody-antigen-antibody(HRP) “sandwich “ complex, the colorless Chromogens are hydrolyzed by the bound HRP conjugate to a blue-colored product. The blue color turns yellow after stopping the reaction with sulfuric acid. The amount of color intensity can be measured and is proportional to the amount of antigen captured in the wells, and to the sample respectively. Wells containing samples negative for HBeAg remain colorless.


PRINCIPLE

 

This kit is an enzyme -linked immunosorbent assay (ELISA) for qualitative detection of hepatitis B virus e antigen (HBeAg) in human serum or plasma. It is intended for use in clinical laboratories for diagnosis and management of patients related to infection with hepatitis B virus. HEPATITIS B VIRUS E ANTIGEN ELISA. One-Step Incubation, Double Antibody Sandwich Principle

 

 

REAGENT

 

Materials provided with the kits:

 

  1.  Negative Control: 0.5ml HBeAg Negative Control.
  2.  Positive Control: 0.5ml HBeAg Positive Control.
  3.  Enzyme Conjugate:.6 ml containing HRP-conjugated-anti-HBe
  4.  Wash Buffer Concentrate (20 x): 20 ml for 96 tests. The buffer should be diluted 20 times with distilled water before use.
  5.  Substrate Solution A: 6 ml HRP Substrate for 96 tests.
  6.  Substrate Solution B: 6 ml TMB Chromagen Substrate for 96 tests.
  7.  Stop Solution: One bottle of 6 ml 2N Sulfuric Acid

 

Materials required but not provided:

  1.  Precision pipettes: 0.02, 0.05, 0.10, 0.15, 0.20, and 1.0 ml.
  2.  Disposable pipette tips.
  3.  Distilled water.
  4.  Humidified Box capable of maintaining 37°C
  5.  Absorbent paper or paper towel.
  6.  Micro titer plate or strip-well washer
  7.  Micro titer plate reader.
 
 
ORIENT NEW LIFE MEDICAL CO., LTD.
Contact: Jerry Meng
Email: Jerry @ newlifebiotest .com
Tel. +86 18657312116
SKYPE enetjerry
 

Contact Details
Orient New Life Medical Co.,Ltd.

Contact Person: Jerry Meng

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