Payment & Shipping Terms:
|Category:||Drug Test Kit||Format:||Dipcard|
|Specimen:||Urine||Testing Time:||5-15 Minutes|
|Shelf Life:||24 Months||Application:||Buprenorphine|
BUP Rapid Diagnostic Test/ Buprenorphine Rapid Diagnostic Test
The BUP Rapid Test Device (Urine) is a rapid visual immunoassay for the qualitative, presumptive detection of Buprenorphine in human urine specimens at the cut-off concentrations listed below:
The BUP One Step Buprenorphine Test Strip (Urine) is an immunoassay based on the principle of competitive binding. Drugs which may be present in the urine specimen compete against the drug conjugate for binding sites on the antibody.
During testing, a urine specimen migrates upward by capillary action. Buprenorphine, if present in the urine specimen below 10 ng/mL, will not saturate the binding sites of antibody-coated particles in the test. The antibody-coated particles will then be captured by immobilized Buprenorphine conjugate and a visible colored line will show up in the test line region. The colored line will not form in the test line region if the Buprenorphine level exceeds 10 ng/mL because it will saturate all the binding sites of anti-Buprenorphine antibodies.
A drug-positive urine specimen will not generate a colored line in the test line region because of drug competition, while a drug-negative urine specimen or a specimen containing a drug concentration lower than the cut-off will generate a line in the test line region.
To serve as a procedural control, a colored line will always appear at the control line region, indicating that proper volume of specimen has been added and membrane wicking has occurred.
DIRECTIONS FOR USE
Bring tests, specimens, and/or controls to room temperature (15-30°C) before use.
Remove the test from its sealed pouch, and place it on a clean, level surface. Label the test with patient or control identification. For best results, the assay should be performed within one hour.
Using the provided disposable pipette, transfer 3 drops of specimen (approximately 120 µL) to the specimen well (S) of the device and start the timer.
Avoid trapping air bubbles in the specimen well (S), and do not add any solution to the result area.
As the test begins to work, color will migrate across the membrane.
Wait for the colored band(s) to appear. The result should be read at 5 minutes. Do not interpret the result after 8 minutes
INTERPRETATION OF RESULTS
NEGATIVE:* A colored line appears in the Control region (C) and colored lines appears in the Test region (T). This negative result means that the concentrations in the urine sample are below the designated cut-off levels for a particular drug tested.
*NOTE: The shade of the colored lines(s) in the Test region (T) may vary. The result should be considered negative whenever there is even a faint line.
POSITIVE: A colored line appears in the Control region (C) and NO line appears in the Test region (T). The positive result means that the drug concentration in the urine sample is greater than the designated cut-off for a specific drug.
INVALID: No line appears in the Control region (C). Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for Control line failure. Read the directions again and repeat the test with a new test card. If the result is still invalid, contact your manufacturer.
A procedural control is included in the test. A line appearing in the control region
(C) is considered an internal procedural control. It confirms sufficient specimen volume, adequate membrane wicking and correct procedural technique.
Control standards are not supplied with this kit. However, it is recommended that positive and negative controls be tested as good laboratory practice to confirm the test procedure and to verify proper test performance.
The specimen correlation study was performed on 226 urine specimens. 56 positive urine specimens and 170 negative urine specimens were confirmed by LC/MS. These specimens were randomized and tested using the BUP One Step Buprenorphine Test Strip (Urine). Specimens were rated as either positive or negative at 5minutes. The test results are shown in Table 1.
Table 1: Specimen Correlation
|BUP One Step Test Strip||+||-|
Positive agreement with LC/MS: 55/(55+1) = 98%
Negative agreement with LC/MS: 168/(168+2) = 99%
Total agreement with LC/MS: (55+168)/(55+1+2+168) = 99%
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|Email:||Jerry @ newlifebiotest .com|
Contact Person: Jerry Meng